DOT Drug Testing Locations in California Near You

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12,500 Drug Testing Locations.

If you work in the transportation industry in California, you may be wondering where to go for a convenient and reliable drug test to comply with the regulations of the Department of Transportation (DOT) drug testing program.

Just sign up and we’ll get you in to a testing center in your zip code.

DOT Compliant Drug Testing Enrollment Prices

At Fleet Drug Testing, we’re proud to offer full-service, DOT-compliant drug testing solutions for employers and safety-sensitive employees at locations throughout California. Whether you’re in San Diego, Los Angeles, San Francisco, Fresno, Sacramento or elsewhere, there’s a drug testing center near you.

Work with our team of experienced professionals can give you peace of mind knowing that your test results will be accurate and that you’ll be in compliance with DOT regulations.

Enroll today to get started, or contact us with any questions that you may have.

We offer variety of products that comply with Department of Transportation CFR Part 382 & 40.


  • ✔️ Certificate of Enrollment
  • ✔️ Online Access to Your Account
  • ✔️ Medical Review
  • ✔️ Nationwide Collection Sites


  • ✔️ Certificate of Enrollment
  • ✔️ Online Access to Your Account
  • ✔️ Medical Review
  • ✔️ Nationwide Collection Sites
  • ✔️ Pre-Employment Drug Test
  • ✔️ Random Drug Testing


  • ✔️ Online Access to Your Account


  • ✔️ Supervisor Role Training
  • ✔️ Alcohol & Drug Test Training
  • ✔️ Training Certificate

DOT Drug Testing Requirements

The DOT’s drug testing program is a crucial aspect of ensuring the safety of everyone on the road, air, or rail. The main thrust of these regulations requires certain “safety-sensitive” employees in the transportation industry to undergo drug testing. This includes employees who:

  • Operate commercial motor vehicles, such as trucks, buses, and certain types of heavy equipment.
  • Any employee whose performance can affect the safety of others, such as air traffic controllers or engineers

For these employees, the DOT’s testing program applies at different times during their employment, whether they work for themselves or a larger company. According to DOT regulations, employees must be tested in the following situations:

  • Pre-Employment – Before starting a safety-sensitive job, employees must take a drug and alcohol test. If the test shows positive results, the employee may not get the job.

  • Post-Accident – Whenever an employee is involved in a work-related accident or incident that causes serious injuries, fatalities, or property damage, they must take a drug and alcohol test.

  • Random testing – Employees in safety-sensitive positions can be randomly chosen for drug and alcohol testing as per DOT regulations. Employers use a computer-based system to select employees for testing, and at least 50% of employees are tested for drugs and 10% for alcohol within a year.

  • Reasonable Suspicion – If there is a reasonable belief or suspicion that an employee is under the influence of drugs or alcohol, DOT regulations require the employee to undergo testing.

  • Return to Duty – After testing positive for drugs or alcohol, employees must complete a substance abuse treatment program and pass a “return-to-duty” test before they can resume their safety-sensitive duties.


What do Drug Tests Screen For

DOT drug tests also screen for several types of drugs, including:

  • Marijuana
  • Cocaine
  • Opiates
  • Amphetamines
  • Phencyclidine (PCP)

Protocols for DOT Drug Testing in California

One of the DOT drug testing program’s main points is ensuring their employees are tested at certified drug testing facilities. These facilities must follow strict protocols to maintain compliance with the regulations and ensure the accuracy of the results. Here are some of the protocols for DOT drug testing in California:

  • Chain of custody – California requires strict chain-of-custody procedures for DOT drug testing. The collection, handling, and transportation of specimens must be documented at every step to ensure the integrity of the testing process.
  • Testing methods – The DOT drug test in California is typically a urine test, although other methods, such as saliva or hair testing, may be used in certain circumstances.
  • Medical Review Officer (MRO) – Employers must designate a Medical Review Officer (MRO) to oversee the testing process and interpret the results. The MRO must be licensed in California and meet the qualifications outlined by the DOT.
  • Employee notification – Employees must be notified in writing of their rights and responsibilities related to DOT drug testing. They must also be informed of the consequences of a positive drug test result.

It is important for employers and employees in California to follow these protocols to ensure that DOT drug testing is conducted fairly, consistently, and legally compliant.

What to Look For in a California Testing Location

For employees, having to take a drug test is sometimes stressful enough, but finding a location near them can sometimes add to the stress. While there are a number of facilities throughout California, our team of experienced professionals at Fleet Drug Testing can handle the process of searching for a location that works for you or your employer.

That said, when looking for a testing location, it’s always good to consider the following:


The testing facility should be certified by the Substance Abuse and Mental Health Services Administration (SAMHSA) and meet all the requirements for DOT drug testing in California.


Look for a testing facility that has experience conducting DOT drug tests and other drug tests, such as pre-employment and random drug tests.


Check the testing facility's reputation by reading reviews or asking for recommendations from other employers or individuals who have used their services.


Choose a testing facility that is convenient and accessible for you or your employees. Ideally, it should be located near your workplace or easily accessible by public transportation.

Testing methods

Ensure that the testing facility uses appropriate testing methods, such as urine, saliva, or hair testing, as required by the DOT regulations.

Turnaround time

Confirm the testing facility's turnaround time for results and make sure it is acceptable for your needs.


Choose a testing facility that takes privacy and confidentiality seriously and has policies and procedures in place to protect the confidentiality of test results.

We Have DOT Drug Testing Locations in California Near You

If you are in need of a DOT drug test in California, consider working with our team at Fleet Drug Testing. With our expertise in DOT drug testing, we can help you locate a certified testing facility near you, ensure compliance with all DOT regulations, and provide accurate and reliable drug test results that can keep you moving.

We can simplify the testing process and ensure compliance with regulations, all the while saving you time and money. if you’re interested in joining our drug testing consortium, reach out to our team for a free consultation to learn more and get started today.