Fleet FMCSA Drug Testing

Simple. Safe.


DOT-Compliant Drug & Alcohol Testing, Consortium and Clearinghouse:

DOT Drug Testing Locations in Illinois Near You

ENROLL in our DOT FMCSA testing program to assure compliance.

No matter where you are, there’s a testing location nearby ready to serve you.

DOT Compliant Drug and Alcohol Testing Services for CDL Drivers

Whether you’ve just obtained your CDL license or are an employer with new drivers, you must comply with DOT/FMCSA Drug and Alcohol Testing rules and regulations

Owner-operators or “for hire” CDL license holders are required to undergo testing through a Third-Party Administrator (C/TPA) such as Fleet Drug Testing before driving a commercial vehicle. They are not permitted to manage testing themselves.

At Fleet Drug Testing we manage everything for you. Simply enroll into our FMCSA drug & alcohol testing consortium and we will ensure your DOT testing requirements are fulfilled in a cost-effective manner.

FMCSA Drug Testing Requirements for Owner-Operator ("For Hire") CDL Drivers

Meeting these requirements is as simple as joining our testing program. We offer enrollment into a consortium which simplifies the compliance process by providing pre-employment testing, random testing and more. This ensures that all FMCSA testing requirements are met in a cost-effective and efficient manner.

FMCSA Drug Testing Requirements for Employers

We offer comprehensive services to help trucking and other CDL employers fulfill these FMCSA requirements. From random testing selection, sample collection, testing, record keeping, and reporting we ensure compliance in a cost-effective and efficient manner.

Need a Testing Site Now?

We’ve Got You Covered!

Testing Locations:

Enroll Now. DOT Compliant Drug & Alcohol Testing Consortium Prices

Choose from our testing programs that comply with Department of Transportation CFR Part 382 & 40.


Per Year
  • Certificate of Enrollment
  • Online Access to Your Account
  • Medical Review
  • Nationwide Collection Sites


Per Year
  • Certificate of Enrollment
  • Online Access to Your Account
  • Medical Review
  • Nationwide Collection Sites
  • Pre-Employment Drug Test
  • Random Drug Testing


  • Online Access to Your Account


  • Supervisor Role Training
  • Alcohol & Drug Test Training
  • Training Certificate

Questions? Give us a call at (888) 709-5029, M-F 8am to 5pm (PDT)

We Provide and Manage All Federally Mandated Testing Requirements:

Pre-Employment Drug Testing

Before operating a commercial motor vehicle (CMV) for the first time, a truck driver or other CDL driver must undergo a drug test and receive a negative test result. Employers & owner/operators must ensure that this test is conducted.

Random Drug and Alcohol Testing

Owner/operators must participate in a DOT-approved random drug and alcohol testing program. The testing rate for random drug testing is 50% per year, while the testing rate for random alcohol testing is 10% per year. Employers have the option of joining or creating their own pool.

Post-accident Drug and Alcohol Testing

If a CDL driver is involved in an accident that meets certain criteria–fatalities, injuries requiring immediate medical attention, or towing of any vehicle–that driver may be required to undergo drug and alcohol testing.

Reasonable Suspicion Drug and Alcohol Testing

If you have reasonable suspicion to believe that a driver is using drugs or alcohol while on duty, the driver may be required to undergo drug and alcohol testing.

Return-to-Duty Drug and Alcohol Testing

If a driver has violated the DOT drug and alcohol testing regulations, he or she must undergo a return-to-duty drug and/or alcohol test before returning to safety-sensitive duties.

Follow-up Drug and Alcohol Testing

If a driver has violated the DOT drug and alcohol testing regulations, he or she may be subject to follow-up testing for up to five years.

Stay Compliant the Easy Way

  • Simplify the drug testing process – Our drug testing consortium can handle all aspects of the drug testing process, including specimen collection, laboratory testing, and result reporting. This can simplify the process for owner-operators and ensure compliance with DOT regulations.
  • Reduce costs – By pooling resources with other companies and owner-operators, consortium members can benefit from reduced drug testing costs. Our consortium can also negotiate lower rates with testing facilities and pass those savings on to our members.
  • Ensure compliance – Probably the biggest benefit to our drug testing consortium is that it can help ensure compliance with all DOT drug and alcohol testing regulations. This can include providing guidance on testing frequency, ensuring proper specimen collection procedures, and maintaining accurate records. All of these are vital for single-vehicle owner-operators.
  • Provide expert support – The staff of our drug & alcohol testing consortium can provide expert support and guidance on all aspects of the drug testing process. This can include answering questions about testing requirements, providing training on drug and alcohol testing procedures, and helping owner-operators navigate any compliance issues.
  • Increase accountability – Our consortium members are held accountable for complying with DOT drug and alcohol testing regulations. This can help ensure a level playing field for all owner-operators and reduce the risk of non-compliance.

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Located in the heartland of the country, Illinois sees its fair share of traffic from large commercial trucks and other vehicles. According to data from the Illinois Department of Transportation, these trucks traveled nearly 13,000 miles on Illinois roads in 2022. Fleet Drug Testing provides DOT-compliant drug testing services for employers and employees in critical roles throughout Illinois, spanning from Champaign to Chicago to Springfield. Whether you’re traveling on Interstate 294 or Interstate 70, our experienced team ensures precise test results and adherence to DOT regulations all across the country. If you want to learn more about our programs or start the enrollment process, just get in touch with us. We’re here to help you stay on the road with peace of mind.

What Are the DOT Drug Testing Requirements for Illinois Transportation Employees?

Knowing and adhering to the drug testing regulations set by the federal Department of Transportation (DOT) is crucial for securing the safety of everyone involved in transportation by road, air, or rail. These rules primarily apply to “safety-sensitive” employees in the transportation industry who must undergo drug testing. This includes employees operating:
  • Commercial trucks, buses, trains, airplanes, and heavy equipment
These regulations also apply to those in roles that can impact the safety of others, such as:
  • Air traffic controllers
  • Engineers
These testing requirements cover various employees within the transportation sector, and the DOT specifies when drug tests are necessary. The situations requiring drug testing include:
  • Pre-employment testing
  • Post-accident testing
  • Random testing at periodic intervals
  • Testing based on reasonable suspicion
  • Testing before an employee returns to duty after a previous positive test result
DOT drug tests screen for specific types of drugs, including marijuana, cocaine, opiates, amphetamines, and phencyclidine (PCP). In addition to drug testing, employees are also subject to periodic alcohol testing, especially after an accident. It’s important to understand that there are specific guidelines to be followed by individuals undergoing testing, as well as guidelines for employers, laboratories, Medical Review Officers (MROs), and other entities involved in the testing process.

What is the DOT Testing Process in Illinois?

The process for DOT testing in Illinois is the same as it is anywhere else. It involves the following procedure, which ensures reliable methods and results:
  • Employers inform employees about the drug testing process, including the time and location of the test.
  • A trained collector at the testing site obtains a urine sample from the employee. They carefully label and seal the sample to ensure it cannot be tampered with.
  • The collected sample is divided into two containers: the primary and split specimens. The primary specimen is sent to a certified laboratory for analysis, while the split specimen is retained at the collection site as a precaution in case of any result discrepancies.
  • At the certified laboratory, the primary specimen undergoes testing using a method called immunoassay. If the result shows positive for drugs, a more precise test known as gas chromatography/mass spectrometry (GC/MS) is performed to confirm the finding.
  • A Medical Review Officer (MRO) thoroughly examines the test results and contacts the employee if there is a positive outcome. During this communication, the MRO inquires about any prescription medications the employee may be taking, which could have influenced the test results. If the employee has a valid medical explanation, the result is reported as negative.
  • The MRO then informs the employer of the test results. If a positive result is indicated, the employee must immediately be removed from safety-sensitive duties, and the employer may need to report the positive outcome to the DOT.
  • Employees who test positive are required to undergo a substance abuse evaluation and participate in a follow-up testing program. They must obtain a negative result on a return-to-duty test before being allowed to resume their safety-sensitive duties.
Following these procedures ensures that the DOT-compliant drug testing process is conducted accurately and in accordance with regulations in Illinois. Still, it’s important to understand what to look for in a testing location.

What Should Be Looked For in a DOT Testing Location in Illinois?

Ensuring compliance with DOT regulations is crucial for your job, and that includes selecting a reliable testing location. Working with our team makes it hassle-free as we ensure the facility meets all DOT regulations and delivers accurate, fast results. Here are key factors to look for in an Illinois testing location:
  • Certification – Our services ensure the facility is SAMHSA-certified and meets all DOT drug testing requirements in Illinois.
  • Experience – Opt for a location with experience in conducting DOT drug tests and other types of drug testing.
  • Reputation – Check online reviews and ask for recommendations to ensure the facility’s reliability.
  • Location – Our drug testing enrollment programs prioritize convenient and easily accessible facilities, ideally close to your workplace or daily route.
  • Testing methods – Choose a location knowledgeable about the appropriate testing methods mandated by DOT, such as urine or saliva testing.
  • Turnaround time – We verify the facility’s turnaround time for providing test results to fit your needs.
  • Confidentiality – Our team selects facilities that prioritize privacy and have measures to protect the confidentiality of test results.
Undergoing drug testing is essential for the safety of transportation industry workers and the public sharing the roads with them. Following DOT regulations ensures you maintain your license. Working with our team guarantees compliance with drug testing regulations while making the entire process easy and convenient. The DOT drug testing program offers numerous advantages for transportation companies operating in Illinois. By working with our team at Fleet Drug Testing, we simplify the process of finding convenient testing locations and meeting all DOT drug testing program requirements.

Keep Moving With Fleet Drug Testing

If your business relies on transportation in any part of Illinois, staying compliant with DOT regulations is a must. Access to convenient drug testing locations across the Bluegrass State is crucial for you and your employees. With our programs at Fleet Drug Testing, we ensure you stay updated on DOT regulations, allowing your business to keep moving smoothly. Get in touch with us today to begin or explore our programs to find the best fit for your needs.