Fleet FMCSA Drug Testing

Simple. Safe.


DOT-Compliant Drug & Alcohol Testing, Consortium and Clearinghouse:

DOT Drug Testing Locations in Colorado Near You

ENROLL in our DOT FMCSA testing program to assure compliance.

No matter where you are, there’s a testing location nearby ready to serve you.

DOT Compliant Drug and Alcohol Testing Services for CDL Drivers

Whether you’ve just obtained your CDL license or are an employer with new drivers, you must comply with DOT/FMCSA Drug and Alcohol Testing rules and regulations

Owner-operators or “for hire” CDL license holders are required to undergo testing through a Third-Party Administrator (C/TPA) such as Fleet Drug Testing before driving a commercial vehicle. They are not permitted to manage testing themselves.

At Fleet Drug Testing we manage everything for you. Simply enroll into our FMCSA drug & alcohol testing consortium and we will ensure your DOT testing requirements are fulfilled in a cost-effective manner.

FMCSA Drug Testing Requirements for Owner-Operator ("For Hire") CDL Drivers

Meeting these requirements is as simple as joining our testing program. We offer enrollment into a consortium which simplifies the compliance process by providing pre-employment testing, random testing and more. This ensures that all FMCSA testing requirements are met in a cost-effective and efficient manner.

FMCSA Drug Testing Requirements for Employers

We offer comprehensive services to help trucking and other CDL employers fulfill these FMCSA requirements. From random testing selection, sample collection, testing, record keeping, and reporting we ensure compliance in a cost-effective and efficient manner.

Need a Testing Site Now?

We’ve Got You Covered!

Testing Locations:

Enroll Now. DOT Compliant Drug & Alcohol Testing Consortium Prices

Choose from our testing programs that comply with Department of Transportation CFR Part 382 & 40.


Per Year
  • Certificate of Enrollment
  • Online Access to Your Account
  • Medical Review
  • Nationwide Collection Sites


Per Year
  • Certificate of Enrollment
  • Online Access to Your Account
  • Medical Review
  • Nationwide Collection Sites
  • Pre-Employment Drug Test
  • Random Drug Testing


  • Online Access to Your Account


  • Supervisor Role Training
  • Alcohol & Drug Test Training
  • Training Certificate

Questions? Give us a call at (888) 709-5029, M-F 8am to 5pm (PDT)

We Provide and Manage All Federally Mandated Testing Requirements:

Pre-Employment Drug Testing

Before operating a commercial motor vehicle (CMV) for the first time, a truck driver or other CDL driver must undergo a drug test and receive a negative test result. Employers & owner/operators must ensure that this test is conducted.

Random Drug and Alcohol Testing

Owner/operators must participate in a DOT-approved random drug and alcohol testing program. The testing rate for random drug testing is 50% per year, while the testing rate for random alcohol testing is 10% per year. Employers have the option of joining or creating their own pool.

Post-accident Drug and Alcohol Testing

If a CDL driver is involved in an accident that meets certain criteria–fatalities, injuries requiring immediate medical attention, or towing of any vehicle–that driver may be required to undergo drug and alcohol testing.

Reasonable Suspicion Drug and Alcohol Testing

If you have reasonable suspicion to believe that a driver is using drugs or alcohol while on duty, the driver may be required to undergo drug and alcohol testing.

Return-to-Duty Drug and Alcohol Testing

If a driver has violated the DOT drug and alcohol testing regulations, he or she must undergo a return-to-duty drug and/or alcohol test before returning to safety-sensitive duties.

Follow-up Drug and Alcohol Testing

If a driver has violated the DOT drug and alcohol testing regulations, he or she may be subject to follow-up testing for up to five years.

Stay Compliant the Easy Way

  • Simplify the drug testing process – Our drug testing consortium can handle all aspects of the drug testing process, including specimen collection, laboratory testing, and result reporting. This can simplify the process for owner-operators and ensure compliance with DOT regulations.
  • Reduce costs – By pooling resources with other companies and owner-operators, consortium members can benefit from reduced drug testing costs. Our consortium can also negotiate lower rates with testing facilities and pass those savings on to our members.
  • Ensure compliance – Probably the biggest benefit to our drug testing consortium is that it can help ensure compliance with all DOT drug and alcohol testing regulations. This can include providing guidance on testing frequency, ensuring proper specimen collection procedures, and maintaining accurate records. All of these are vital for single-vehicle owner-operators.
  • Provide expert support – The staff of our drug & alcohol testing consortium can provide expert support and guidance on all aspects of the drug testing process. This can include answering questions about testing requirements, providing training on drug and alcohol testing procedures, and helping owner-operators navigate any compliance issues.
  • Increase accountability – Our consortium members are held accountable for complying with DOT drug and alcohol testing regulations. This can help ensure a level playing field for all owner-operators and reduce the risk of non-compliance.

Get DOT Compliance Drug Testing

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In order to work in certain positions in the transportation industry, it’s crucial to follow the regulations set by the Department of Transportation (DOT), especially their Drug and Alcohol Testing Program. This program, which applies to these employees throughout Colorado, is designed to ensure the safety of everyone on the road, especially those operating large commercial vehicles.

However, complying with these regulations can be difficult. In addition to ensuring that you or your employees are free from drugs and alcohol, finding a nearby location for drug testing can also be a challenge.

Luckily, Fleet Drug Testing is here to help you stay on top of DOT regulations. Our team of experienced professionals offers a wide range of drug testing services and programs. We can assist you in finding a convenient drug testing site in Colorado, whether you’re passing through Boulder, Denver, Aspen, or other cities.

No matter where your journey takes you in Colorado, our team can help you find a drug testing location that will keep you compliant. Contact us today to get started or visit our enrollment page to explore the programs that can suit your needs.

What is a Safety Sensitive Employee?

Part of the DOT’s drug testing program involves those employees that require testing. Specifically, all “safety-sensitive” employees require drug testing under these regulations. According to the DOT, a safety-sensitive employee is any worker that:

  • Drives commercial motor vehicles like trucks, buses, and specific heavy equipment, or;
  • Whose job performance can impact the safety of others

In general, any of the following could be considered a “safety-sensitive” employee, and as a result, are subject to the DOT’s drug testing regulations:

  • Truck drivers
  • Bus drivers
  • Airline pilots
  • Air traffic controllers
  • Train engineers
  • Railroad conductors
  • Ship captains and crew
  • Construction equipment operators
  • Pipeline operators
  • Emergency vehicle drivers
  • Mass transit operators (subway, tram, light rail, etc.)

DOT Drug Testing Requirements For Colorado Employees

In Colorado, complying with the federally regulated DOT drug testing program is a requirement to remain licensed. As it relates specifically to the program, testing is done under the following conditions for these employees:

  • Pre-employment testing – Before being hired for a safety-sensitive job, employees must undergo a drug and alcohol test. If the results are positive, they may not be hired.
  • Random testing – Safety-sensitive employees are subject to random drug and alcohol testing according to DOT regulations. Employers can use a system that randomly picks employees for testing. They have to do drug testing on at least half of the employees and alcohol testing on at least 10% of them within a one-year period.
  • Reasonable suspicion testing – If there is a reasonable belief or suspicion that an employee is under the influence of drugs or alcohol, they must undergo testing as mandated by DOT regulations.
  • Post-accident testing – In cases where a work-related accident or incident results in a fatality, serious injury, or property damage, the employee involved must undergo drug and alcohol testing.
  • Return-to-duty testing – After a positive drug or alcohol test result, the employee must complete a substance abuse treatment program and pass a “return-to-duty” test before being allowed to resume safety-sensitive duties.
  • Follow-up testing – Employees who tested positive for drugs or alcohol and have returned to their safety-sensitive duties must undergo follow-up testing as required.

The DOT mandates drug testing using urine samples, which screen for five types of drugs: marijuana (THC), cocaine, amphetamines, opiates, and Phencyclidine (PCP). These tests must be conducted at certified facilities, which can be found throughout Colorado.

What Happens If You Don’t Follow DOT Drug Testing Regulations?

It should come as no surprise that not following DOT regulations can have significant negative consequences for your business, whether you manage a fleet of trucks or operate your own vehicle. To that end, it’s crucial to not use drugs or alcohol while driving, but just as important to follow other regulations, such as using a DOT-approved certified lab for testing. Failure to do so can cast doubt on the validity of the testing results and put your compliance at risk.

That’s why using our services is so convenient: we handle all of the details to make sure you stay up to date and compliant with regulations, allowing you to focus on your business.

Still, these regulations are in place for a reason – to prioritize the safety of everyone involved. When you or your company fail to comply with them, there are serious repercussions to consider:

  • Financial consequences – Non-compliance can result in financial penalties, loss of contracts, and potential legal actions. Additionally, the DOT may increase scrutiny on your operations, making it challenging to obtain permits or licenses in the future.
  • Employment consequences – For employees, failing a drug test or disregarding DOT regulations can lead to suspension or termination of employment, loss of commercial driving privileges, and difficulties in finding future employment within the transportation industry.

Advantages of a DOT Testing Program

There are many advantages to having a certified DOT drug testing program. While it can ensure your compliance and allow you to keep your business moving, there are other, even more important advantages, like:

  • Improved safety – Following DOT regulations enhances safety for everyone involved in the transportation industry, including your employees and the public. By adhering to these regulations, you create a safer work environment and reduce the risk of accidents and injuries.
  • Promotes compliance – Complying with DOT regulations demonstrates your commitment to following the rules and regulations set by the government. This promotes a culture of compliance within your company and helps you avoid penalties and legal issues that may arise from non-compliance.
  • Safeguards reputation – Adhering to DOT regulations helps protect your company’s reputation. By maintaining compliance, you establish yourself as a responsible and trustworthy business, which can enhance your credibility in the industry and attract more customers and business opportunities.
  • Employee well-being – Following DOT regulations, including drug testing programs, ensures the health and well-being of your employees. By conducting regular drug tests, you can identify and address any substance abuse issues promptly, helping your employees maintain a healthy and safe working environment.

Stay Compliant in Colorado With Fleet Drug Testing

When your business depends on staying compliant with DOT regulations, Fleet Drug Testing is here to help. We understand that finding a convenient drug testing location that meets all DOT regulations can be challenging. Our testing programs remove the guesswork and provide access to certified labs throughout Colorado, making the process smoother for you. By staying in compliance with DOT regulations, you not only prioritize safety but also keep your business running smoothly.

Contact us today to get started and ensure that you are fully compliant with DOT regulations.